Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jie Tang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 07:37:39
Improve the protein purification.
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1947027_sequence (Version 1) |
Description
Long description: TEV protease may bring some impure proteins. We use intein to replace the TEV site. The M. xenopi GyrA intein provides a paradigm for a minimal protein splicing element. Intein can be cleaved by DTT. DTT as reducing agent protects the free thiol groups of the protein from oxidation and so it doesn???t bring foreign substance. As a result, intein can achieve a better effect of purification than TEV protease.Notes
GS linker is used to construct the fusion protein. Amilcp does not have a termination codon.Source
Protein splicing elements (termed inteins) were described as in-frame insertions in the Saccharomyces cerevisiae VMA1 gene.| Access | Instance | Definition |
| public public public public public | BBa_K592009 BBa_K1486004 BBa_K1947002 BBa_K1486004 BBa_K1159201 | amilCP BBa_K1486004 Intein BBa_K1486004 BBa_K1159201 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K592009 BBa_K1486004 BBa_K1947002 BBa_K1486004 BBa_K1159201 | 1,669 678,707 714,1316 1325,1354 1363,1401 | amilCP BBa_K1486004 Intein BBa_K1486004 BBa_K1159201 |