Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: claire raynaud
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-15 02:18:05
desABCD cluster producing biosynthesis pathway to make Desferrioxamine B
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K1951011_sequence (Version 1) |
Description
Desferrioxamine B is a siderophore produced in Streptomyces coelicolor to catch vital irons on the extracellular medium. In consequence, this molecule which have a greatly high affinity for iron is secreted in the external medium and bring back inside the bacteria full of iron mol??cules.Previous work showed that siderophore which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Especifically, many articles showed a high affinity of Desferrioxamine B ( produce by Streptomyces coelicolor) for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. E. coli was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive, considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate trasnscription using the control of an inductible promotor (pBAD/araC).
Our strategy for the siderophore mobilisation results in two main steps :
1. Transformation of E.Coli with an inductible plasmide holding the des cluster
2. Induction and production of the desferrioxamine B
Notes
There is no any design consideration for this biobrick.Source
We used 7 previous biobricks to design this one :- BBa_B0034
- BBa_I0500
- BBa_K1951000
- BBa_K1951001
- BBa_K1951002
- BBa_K1951003
| Access | Instance | Definition |
| public public public public public public | BBa_I0500 BBa_B0034 BBa_K1951000 BBa_K1951001 BBa_K1951002 BBa_K1951003 | pBad/araC BBa_B0034 BBa_K1951000 BBa_K1951001 BBa_K1951002 BBa_K1951003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 BBa_B0034 BBa_K1951000 BBa_K1951001 BBa_K1951002 BBa_K1951003 | 1,1210 1219,1230 1237,2682 2689,3933 3940,4497 4504,6294 | pBad/araC BBa_B0034 BBa_K1951000 BBa_K1951001 BBa_K1951002 BBa_K1951003 |