Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_K1954006_sequence (Version 1)
|
Description
N-acetylglutamate synthetase (NAGS) catalyzes the first step of the linear biosynthetic pathway of L-arginine as follows:
acetyl-coA + L-glutamate = coA + N-acetyl-L-glutamate
This sequence is the open reading frame of the feedback resistant mutant PT2M217 NAGS which is not responsive to the inhibition by L-arginine present in the endogenous system.
Notes
One restriction site was removed from the source sequence by silent mutagenesis as follows:
PstI 421: Leu 121 (CTG) 46.9 % -> Leu (TTA) 15.2 %
Source
AF008119.1