BBa_K1954006

BBa_K1954006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1954006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kamil Żmijewski
Date created: 2016-10-18 11:00:00
Date modified: 2016-10-25 07:13:55

Feedback-resistant N-acetylglutamate synthetase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1954006_sequence (Version 1)

Description

N-acetylglutamate synthetase (NAGS) catalyzes the first step of the linear biosynthetic pathway of L-arginine as follows:

acetyl-coA + L-glutamate = coA + N-acetyl-L-glutamate

This sequence is the open reading frame of the feedback resistant mutant PT2M217 NAGS which is not responsive to the inhibition by L-arginine present in the endogenous system.

Notes

One restriction site was removed from the source sequence by silent mutagenesis as follows:

PstI 421: Leu 121 (CTG) 46.9 % -> Leu (TTA) 15.2 %

Source

AF008119.1

Sequence Annotation Location Component / Role(s)
BBa_I14033
BBa_B0034
frNAGS
BBa_B0014
1,38
39,50
57,1388
1400,1494
promoter feature/promoter
engineered_region feature/BioBrick
feature/cds CDS
feature/stop stop_codon
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1954006/1