Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Syue-Liang Lin
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:16
pTetR+RBS+PelB+F10scFv+C-IgAP+Term
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K195411_sequence (Version 1) |
Description
The leader sequence of C-IgAP is cleaved at the membrane by a signal peptidase, releasing the mature polyprotein into the periplasm. When in the periplasm, the beta-domain of the protein inserts into the outer membrane to form a beta-barrel structure. After formation of the beta-barrel pore, the passenger domain is translocated to the cell surface through the pore.Fusion of a scFv to the IgA protease allowed us to investigate the passage of the chimeric protein through the periplasm, its insertion into the outer membrane and the movement of the N-terminal moiety towards the cell surface.
So we can anchor the F10scFv(antibody) on E.coli 's surface,and catch the influenza A virus.
Notes
The PelB+scFv+C-IgAP didn't have any scar,so we used PCR to In-fusion.Source
This part is composed of pTetR(BBa_R0040),RBS(BBa_B0034),PelB(BBa_J32015),F10scFv(BBa_K195400),C-IgAP(BBa_K195406) and terminator (BBa_B0015)| Access | Instance | Definition |
| public public public public | BBa_R0040 BBa_B0034 BBa_K195408 BBa_B0015 | p(tetR) BBa_B0034 BBa_K195408 BBa_B0015 |