BBa_K1955002

BBa_K1955002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1955002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Justine Hsu

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-17 07:14:28

Leishmania- 3 prime UTR

Details

• Types: DnaRegion
• Roles: terminator; Terminator
• Sequences: BBa_K1955002_sequence (Version 1)

Description

The specific terminator function in Leishmania genome has not been elucidated yet. We selected the 3??? untranslated region of a highly expressed gene, NAGT, to be the transcription terminator and other extra function modifications needed for Leishmania protein expression termination.
This biobrick needs to use with the Leish-5'UTR-HYG that we provide as the regulation of protein expression and also the drug selection system for the Leishmania to perform a complete protein expression system in Leishamnia. Add the protein sequence in between the Leish-5'UTR-HYG and Leish-3'UTR for Leishmania to express your target protein.

Notes

The Leish-3???UTR is a 774 bp DNA sequence, which is directly synthesized by IDT. We acquired the original leishmania genome sequence of NAGT coding region and intergenic region from NCBI (GenBank accession# L11705), and choose the 3???UTR region of the sequence as the transcription terminator and protein expression regulator in our expression vector. Some nucleotides had been changed to avoid the restriction site. The function of the 3???UTR intergenic region was studied and published by Dr. Kwang-Poo Chang (Gene 196 (1997) 49???59).

Source

The Leish-3'UTR sequence originally came from Leishmania genome and is acquired from GenBank.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1955002/1

Member of these Collections

• iGEM Parts Registry