Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nancy Teng
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-06 10:16:32
HydB (C-terminally tagged) subunit of FeFe Hydrogenase
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1957002_sequence (Version 1) |
Description
One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically this is the HydB subunit which is the central subunit. This basic part has a strep-tag attached to its C-terminus for consequent protein assays. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.Notes
This is one of of the three FeFe Hydrogenase subunits. To make the entire FeFe Hydrogenase construct the following BioBricks need to be ligated with BBa_K1957002: BBa_K1957004 and BBa_K1957001.pSB1C3 would not conjugate properly into S.oneidensis, thus the three subunits were ligated using golden gate cloning into the pBAD expression vector.