Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei Wei
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 09:35:51
PbrR -> C. metallidurans
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1958007_sequence (Version 1) |
Description
PbrR is amplified from Cupriavidus metallidurans CH34 (formerly Ralstonia metallidurans) genome.PbrR, together with its homologues in the same bacterium, are the only known lead(II)-specific binding protein found in nature. PbrR binds lead(II) 1000-fold more selectively over other metal ions such as mercury(II), zinc(II), copper(II), nickel(II), and silver(I). Besides lead(II), PbrR also shows the binding capacity of cadmium(II), but slightly lower than that of lead(II). The binding capacity may be concerned with three conserved Cys residues: Cys78, Cys113 and Cys122.
Because the gene encoding PbrR contains a digest site of Pst I (-CTGCAG-), to fit the construction of standard biobrick, we change those site from -CTGCAG- to -CTGCAA- by point mutation.
Notes
None.Source
Cupriavidus metallidurans CH34| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon PbrR coding region TAG stop codon | 1,3 1,438 436,438 | feature/start start_codon feature/cds CDS stop_codon feature/stop |