Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Junyu Chen
Date created: 2016-10-03 11:00:00
Date modified: 2016-10-24 02:02:32
BKT coding sequence fused with Pea transit peptide
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1959003_sequence (Version 1) |
Description
This part is consist of a Pea transit peptide and the coding sequence of beta-carotene ketolase originally derived fromChlamydomonas reinhardtii. Beta-carotene ketolase realizes the convertion of zeaxanthin to astaxanthin, the final step of astaxanthin biosynthesis. Sequence of beta-carotene ketolase has been optimized for better expression in Orazy sativa.
Notes
A pea transit peptide has been functionally fused to the BKT cds. The BKT codon has made some optimization for expression in rice. An Pst I restriction site has been mutated by site-directed mutation.Source
Original sequence can be accessed from Genebank, Genebank accession: AY860820.1.| Sequence Annotation | Location | Component / Role(s) |
| Pst I mutant BKT Pea transit peptide Initiation codon of BKT Initiation codon of Pea transit peptide Termination codon of BKT | 1036,1041 172,1158 1,171 172,174 1,3 1156,1158 | feature/s_mutation point_mutation feature/cds CDS feature/cds CDS feature/start start_codon start_codon feature/start stop_codon feature/stop |