HfsH

BBa_K196003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K196003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laetitia Warny
Date created: 2009-08-11 11:00:00
Date modified: 2015-05-08 01:11:17

HfsH protein from Caulobacter crescentus



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K196003_sequence (Version 1)

Description

Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. Here you have HfsH.

Notes

As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt.

Source

This sequence comes from Caulobacter crescentus.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K196003/1