Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rengwei Liu
Date created: 2016-09-28 11:00:00
Date modified: 2016-09-29 08:40:12
P2 promoter with RBS
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1960900_sequence (Version 1) |
Description
This part is the promoter, P2 with BBa_B0034, a useful RBS.The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the activity of agr P2 promoter about 50-fold.
The cell density at which the agr system is activated is determined by the AgrA-independent activity of P2 promoter
The basal agr P2 promoter activity is high enough to make stochastic effects negligible. (E. Gustafsson et al. 2004)
Notes
The part BBa_I746104 on 2016 DNA Kit Plate is inconsistent, so we had to synthetise this part ourselves.Source
We got the sequence from parts.igem.org, designed the whole sequence and add BioBrick prefix and suffix using SnapGene. The part was synthetised by Sangon Biotech??, Shanghai, China.| Access | Instance | Definition |
| public public | BBa_I746104 BBa_B0034 | BBa_I746104 BBa_B0034 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I746104 BBa_B0034 | 1,96 105,116 | BBa_I746104 BBa_B0034 |