Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 02:23:41
Colicin Ia
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1962000_sequence (Version 1) |
Description
This sequence codes for the full length Colicin Ia bacteriocin (anti-bacterial toxin). Colicin Ia is a protein that is used by species of E. coli in order to kill closely related species of E. coli in certain circumstances e.g. competition. The producer cell normally contains a protein which confers immunity to this toxin, which in this case is (BBA_). And this protein is believed to form a complex with the cytotoxic domain of the bacterocin rendering it inactive. When this bacteriocin is secreted the immunity protein dissociates from the complex leaving the cytotoxic domain active resulting in the pore forming activity of this bacteriocin returning and having effect on the target cells. The pore forming activity of this bacteriocin results in the depolarisation of the bacterial inner membrane resulting in the collapse of the protonmotive force.Notes
Confers to REF[10].Source
Optimised DNA sequence based on back-translation of the protein sequence was designed by Dundee iGEM (2016) and synthesised synthetically by IDT.| Sequence Annotation | Location | Component / Role(s) |
| Colicin Ia Bacteriocin (Toxin) Domain | 1,1881 1351,1881 | CDS feature/cds sequence_feature feature/misc |