BBa_K1962003

BBa_K1962003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1962003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Frank Sargent

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-11 03:58:14

Colicin Ia::Ssp2 Chimera

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1962003_sequence (Version 1)

Description

This part contains an translational fusion protein of the truncated bacteriocin-free version of Colicin Ia (BBa_K1962002) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp2 from Serratia marcescens. Ssp2 is an antibacterial effector normally secreted by the Type VI secretion system (T7SS) in Serratia marcescens and it has peptidoglycan endopeptidase activity, degrading peptidoglycan in the periplasm of the target cell where it cleaves between y-D-glutamic acid and L-mesodiaminopimelic acid in peptidoglycan.

Notes

Compliant with RFC[10].

Source

The sequence encoding Ssp2 was amplified from a Serratia marcescens genomic DNA template and cloned into BBa_K1962002, which had previously been designed and synthesised.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Truncated Colicin Ia	1,1350	feature/cds, CDS
Ssp2	1357,1830	feature/cds, CDS
HA epitope	1831,1863	feature/tag, tag

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1962003/1

Member of these Collections

• iGEM Parts Registry