BBa_K1962008

BBa_K1962008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1962008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Frank Sargent

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-11 07:40:20

Colicin E9::Ssp2 Chimera

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1962008_sequence (Version 1)

Description

This part contains an translational fusion protein between the truncated DNase-domain-lacking version of Colicin E9 (BBa_K1962006) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp2 from Serratia marcescens. Ssp2 is an antibacterial effector normally secreted by the Type VI secretion system (T6SS) by Serratia marcescens and it has peptidoglycan endopeptidase activity.

Notes

Compliant with RFC[10]

Source

The sequence encoding Ssp2 was amplified from a Serratia marcescens genomic DNA template and cloned into BBa_K1962006, which had previously been designed and synthesised.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Truncated E9	1,1356	CDS, feature/cds
Serratia Ssp2	1363,1836	feature/cds, CDS
HA epitope	1837,1869	feature/tag, tag

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1962008/1

Member of these Collections

• iGEM Parts Registry