Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-17 05:25:37
A template for expression of sRNA fusions to the E. coli micC hairpin
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K1963002_sequence (Version 1) |
Description
This is a multi-sequence system for small RNA (sRNA) production in an E. coli host or with co-expression of E. coli Hfq in an alternative host. The system has the proD strong promoter, followed by the micC hairpin, followed by the strin terminator sequence T1/TE. Phosphorylated primers should be used to insert the reverse/complement target sequence of your choice between the promoter and the hairpin sequence.Notes
The sequence is RFC[10] compliant.Source
This was designed de novo and synthesised as a gBlock by IDT for the Dundee_Schools iGEM team in 2016.| Sequence Annotation | Location | Component / Role(s) |
| proD promoter micC loop terminator | 1,193 194,271 272,402 | feature/promoter promoter stem_loop feature/stem_loop sequence_feature feature/misc |