Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-07 02:09:22
A template for expression of sRNA fusions to the Serratia chiA hairpin
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1963003_sequence (Version 1) |
Description
This is a multi-sequence system for small RNA (sRNA) production with co-expression of S. marcescens Hfq. The system has the proD strong promoter, followed by a chiA hairpin sequence from the 3'-end of the S. marcescens chiA gene encoding a chitinase, followed by the strong terminator sequence T1/TE. The use of an S. marcescens chiA hairpin/Hfq pairing should provide very high specificity in chosen chassis organisms. Phosphorylated primers should be used to insert the reverse/complement target sequence of your choice between the promoter and the hairpin sequence. The sRNA should be placed betweeen C-193 and G-194.Notes
The sequence is RFC[10] compliant.Source
This part was synthesised de novo as a gBlock by IDT for the Dundee_Schools iGEM team 2016.| Sequence Annotation | Location | Component / Role(s) |
| proD promoter chiA hairpin terminator | 1,193 194,234 235,368 | feature/promoter promoter stem_loop feature/stem_loop sequence_feature feature/misc |