Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-23 09:17:38
An sRNA system for down-regulation of E. coli fliC encoding flagellin.
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1963004_sequence (Version 1) |
Description
This is a multi-sequence system for small RNA (sRNA) production in an E. coli host - or especially with co-expression of E. coli Hfq. The system is based on the BBa_K1963002 template and has the proD strong promoter, followed by a short antisense sequence targeting the 5' end of the fliC transcript, followed by the E. coli micC hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed betweeen C-193 and T-194. The system should impair motility of E. coli.Notes
This part is RFC[10] compliant.Source
This part was generated by PCR using phosphorylated primers and BBa_K1963002 as template.| Sequence Annotation | Location | Component / Role(s) |
| proD promoter sRNA micC loop terminator | 1,193 194,218 219,296 297,426 | promoter feature/promoter non_covalent_binding_site feature/binding feature/stem_loop stem_loop feature/misc sequence_feature |