Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fatima Ulhuq
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-23 09:18:27
An sRNA system for down-regulation of E. coli fliC encoding flagellin
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1963010_sequence (Version 1) |
Description
This is a multi-sequence system for small RNA (sRNA) production in an E. coli host - or especially with co-expression of E. coli Hfq BBa_K1963000. The system is based on the BBa_K1963002 template and has the proD strong promoter, followed by a short antisense sequence targeting the RBS and 5' end of the fliC transcript, followed by the E. coli micC hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed betweeen C-193 and T-194. The system should impair motility of E. coli.Notes
This part is RFC[10] compliantSource
This part was generated by PCR using phosphorylated primers and BBa_K1963002 as template.| Sequence Annotation | Location | Component / Role(s) |
| proD promoter sRNA micC loop terminator | 1,193 194,213 214,286 287,420 | promoter feature/promoter feature/binding non_covalent_binding_site stem_loop feature/stem_loop sequence_feature feature/misc |