Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fatima Ulhuq
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-18 04:16:20
An sRNA system for down-regulation of Serratia marcescens fliC encoding flagellin.
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1963011_sequence (Version 1) |
Description
This is a multi-sequence system for small RNA (sRNA) production in an Serratia marcescens host - or especially with co-expression of Serratia marcescens Hfq BBa_K1963001. The system is based on the BBa_K1963003 template and has the proD strong promoter, followed by a short antisense sequence targeting the 5' end of the fliC transcript, followed by the S. marcescens chiA hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed betweeen C-193 and T-194. The system should impair motility of S. marcescens.Notes
N/ASource
This part was generated by PCR using phosphorylated primers and BBa_K1963002 as template.| Sequence Annotation | Location | Component / Role(s) |
| proD promoter fliC targeting sRNA chiA loop terminator | 1,193 194,229 230,271 272,389 | promoter feature/promoter non_covalent_binding_site feature/binding stem_loop feature/stem_loop sequence_feature feature/misc |