| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1964000_sequence (Version 1)
|
Description
Codon optimized version of the panD gene from C. glutamicum. Encodes for the aspatarte 1-decarboxylase that catalyzes the chemical reaction of L-aspartate to beta-alanine + CO2. The protein consists 136 amino acid residues with a deduced molecular mass of 14.1 kDa. It belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds.
Notes
While optimized for codon usage in E. coli we had to consider the biobrick restriction sites of EcoRI, PstI, XbaI, SpeI. We manually altered the sequence to avoid these cut sites.
Source
Synthesized DNA Sequence.
