Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nik Franko
Date created: 2016-10-16 11:00:00
Date modified: 2016-10-19 02:56:33
His:CIBN:cLuc:HA
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1965008_sequence (Version 1) |
Description
This part is one of the two components of the dimerazing system which is induced by blue light. We adapted this system for the reconstitution of split luciferase to create a blue-light sensor. To the C-terminus of CIBN, C-terminus of split firefly luciferase was added. Upon induction with light and concequent dimerisation, the split luciferase is reconstituted and regains activity, which results in biolumiescence if luciferin is added.Notes
/Source
Arabidopsis thali and Photinus pyralis (Common eastern firefly)| Sequence Annotation | Location | Component / Role(s) |
| start His CIBN 10 GS linker cLuc GSS linker HA stop | 1,3 4,21 22,528 529,558 559,741 742,750 751,777 778,780 | feature/start start_codon tag feature/tag CDS feature/cds sequence_feature feature/misc CDS feature/cds feature/misc sequence_feature tag feature/tag feature/stop stop_codon |