BBa_K1965011

BBa_K1965011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1965011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Katja Leben

Date created: 2016-10-16 11:00:00

Date modified: 2016-10-18 05:27:08

P5:cLuc

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1965011_sequence (Version 1)

Description

This is a coiled coil peptide attached to C-terminal of split luciferase. The coiled coil paires with AP6; upon dimerization the split luciferase reconstitutes and regains activity, resulting in bioluminescence.

Notes

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Source

cLuc - Photinus pyralis (Common eastern firefly)

P5 - artificial sequence

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GS-linker	103,132	feature/misc, sequence_feature
start	1,3	feature/start, start_codon
P5	4,102	CDS, feature/cds
cLuc	133,315	start_codon, feature/start
HA-tag	325,351	feature/misc, sequence_feature
stop	352,354	feature/stop, stop_codon

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1965011/1

Member of these Collections

• iGEM Parts Registry