BBa_K1965018

BBa_K1965018 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1965018
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nik Franko
Date created: 2016-10-16 11:00:00
Date modified: 2016-10-17 04:53:51

CaM(E31Q,E104Q):cLuc



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1965018_sequence (Version 1)

Description

CaM(E31Q,E104Q):cLuc is a genetic fusion of human calmodulin with two introduced mutation E31Q, E104Q and C terminal fragment of split firefly luciferase. Both mutations are located in EF hands of calmodulin and lowers the construct's sensitivity to calcium ion concentration. The part in combination with BBa_K1965014 (nLuc:M13) reconstitutes luciferase activity upon calcium influx into the cell.

Notes

/

Source

Photinus pyralis (Common eastern firefly) and Homo sapiens (Human)

Sequence Annotation Location Component / Role(s)
start
CaM(E31Q,E104Q)
10GS linker
cLuc
GSG linker
HA
stop
1,3
4,444
445,474
475,657
658,666
667,693
694,696
start_codon feature/start
CDS feature/cds
feature/misc sequence_feature
feature/cds CDS
feature/misc sequence_feature
feature/tag tag
stop_codon feature/stop
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1965018/1