BBa_K1966004

BBa_K1966004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1966004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brandon Wilbanks

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-13 06:25:21

lacPromoter-xylR-PuPromoter-RFP

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1966004_sequence (Version 1)

Description

This part contains RFP and the Pu promoter under its activator for characterization of promoter function. xylR is required for activation of Pu. Part was not shown to function by UTK 2016 iGEM team (reasons unknown).

Notes

Constructed from synthetic DNA via Biobrick assembly

Source

Psuedomonas putida TOL pathway (upper operon)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacPromoter (part BBa_R0011)	1,53	promoter, feature/promoter
RBS (part BBa_B0034)	54,65	feature/rbs, ribosome_entry_site
xylR	66,1766	sequence_feature, feature/dna
T1 Terminator (part BBa_B0010)	1767,1846	feature/misc, sequence_feature
Pu Promoter	1847,2166	feature/promoter, promoter
RBS (part BBa_B0034)	2167,2178	ribosome_entry_site, feature/rbs
RFP	2179,2856	feature/dna, sequence_feature
T1 Terminator (part BBa_B0010)	2857,2936	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1966004/1

Member of these Collections

• iGEM Parts Registry