BBa_K1968000

BBa_K1968000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1968000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Paolo Marangio

Date created: 2016-10-05 11:00:00

Date modified: 2016-10-17 10:52:50

PA1lac0-1 Phytobrick

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1968000_sequence (Version 1)

Description

Derived from the PA1 promoter of bacteriophage T7, PA1lac0-1 is a LacI-dependent promoter and Isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible promoter that has been shown to bring about an 8-fold induction in both E. coli and Synechocystis (Guerrero et al., 2012).


Guerrero, F., Carbonell, V., Cossu, M., Correddu, D., & Jones, P. R. (2012). Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS One, 7(11), e50470. doi: 10.1371/journal.pone.0050470

Notes

The sequence of this part did not need to be re-designed.

Source

Ordered as gblock through IDT

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
PA1lac0-1	5,76	promoter, feature/promoter
Phytobrick Adapter Site A	1,4	sequence_feature, feature/misc
Phytobrick Adapter Site B	77,80	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1968000/1

Member of these Collections

• iGEM Parts Registry