Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Paolo Marangio
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-17 10:52:50
PA1lac0-1 Phytobrick
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1968000_sequence (Version 1) |
Description
Derived from the PA1 promoter of bacteriophage T7, PA1lac0-1 is a LacI-dependent promoter and Isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible promoter that has been shown to bring about an 8-fold induction in both E. coli and Synechocystis (Guerrero et al., 2012).Guerrero, F., Carbonell, V., Cossu, M., Correddu, D., & Jones, P. R. (2012). Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS One, 7(11), e50470. doi: 10.1371/journal.pone.0050470
Notes
The sequence of this part did not need to be re-designed.Source
Ordered as gblock through IDT| Sequence Annotation | Location | Component / Role(s) |
| PA1lac0-1 Phytobrick Adapter Site A Phytobrick Adapter Site B | 5,76 1,4 77,80 | promoter feature/promoter sequence_feature feature/misc sequence_feature feature/misc |