Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Paolo Marangio
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-17 10:54:53
Synechocystis RBS* Phytobrick
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_K1968002_sequence (Version 1) |
Description
RBS* is a ribosome binding site that has been specifically designed to include the anti-Shine Dalgarno (SD) sequence of Synechocystis sp. strain PCC 6803. If no extra bases are added to its sequence, RBS* has an optimal spacing of 9bp between the central base of the core SD sequence (which is base A at position 7 of its sequence) and the first base of the start codon (1). RBS* has been shown to be 5 times stronger than BBa_B0034 in Synechocystis (1), making it the strongest currently available RBS for heterologous gene expression in this cyanobacterium. In E. coli, RBS* is one-third weaker than BBa_B0034.(1) Heidorn, T., Camsund, D., Huang, H. H., Lindberg, P., Oliveira, P., Stensjo, K., & Lindblad, P. (2011). Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol, 497, 539-579. doi: 10.1016/B978-0-12-385075-1.00024-X
Notes
5 random nucleotides (TACTA) were added to the 3??? end of the RBS* sequence in order to allow for easier binding to the ribosome .Source
This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing.| Sequence Annotation | Location | Component / Role(s) |
| Core anti-SD motif Extra 5 bp for easier binding of ribosome to RBS Phytobrick Adapter Site B Phytobrick Adapter Site C RBS* | 9,13 15,19 1,4 20,23 5,19 | non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/misc sequence_feature sequence_feature feature/misc ribosome_entry_site feature/rbs |