BBa_K1968003

BBa_K1968003 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1968003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alejandra

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-11 01:14:51

PglaA inducible promoter: glucoamylase gene promoter (PglaA) from Aspergillus niger

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1968003_sequence (Version 1)

Description

The Aspergillus a-glucoamylase (GlaA) enzyme is involved in starch utilization. The glaA promoter is induced by starch, maltose, and low concentrations of glucose but repressed by xylose (1). Basal transcription of glaA requires only 214 bp upstream of the start codon, while a high-level transcription expression requires the upstream region including -562 and -318 bp in the promoter region. The glaA promoters among different Aspergillus species are conserved.

(1)Fowler, T., Berka, R. M., & Ward, M. (1990). Regulation of the glaA gene of Aspergillus niger. Curr Genet, 18(6), 537-545.

Notes

This part contains Phytobricks overhangs E and C, because this promoter includes the RBS and was the second promoter in our specific plasmid design.

Source

This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). After the part was cloned, its sequence was confirmed by Sanger Sequencing.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
xxxxx	1,5	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1968003/1

Member of these Collections

• iGEM Parts Registry