ErmE

BBa_K1968008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1968008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ibrahim Al-Masoud
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-19 03:05:34

ErmE promoter Phytobrick



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1968008_sequence (Version 1)

Description

A widely used constitutive promoter, ErmE (promoter region of erythromycin-resistance gene) was isolated from Streptomycin (Bibb et al., 1985). It works with related gram positive actinobacteria, such as Rhodococcus. We have this part in pUPD2, a MoClo-compatible level 0 vector with no illegal sites for MoClo assembly standard, GoldenBraid assembly standard and iGEM???s pairwise assembly standard.

References:
Bibb, M.J., Janssen, G.R. and Ward, J.M., 1985. Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene, 38, pp.215-226.

Tong, Y., Charusanti, P., Zhang, L., Weber, T. and Lee, S.Y., 2015. CRISPR-Cas9 based engineering of actinomycetal genomes. ACS synthetic biology, 4, pp.1020-1029.

Notes

The part was integrated in reverse and we advise the user to use the reverse complement sequence for downstream applications.

Source

This part was extracted through PCR from pCRISPR-Cas9 vector (GenBank accession number KR011749) (Tong et al., 2015) and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500 or pUPD2). The sequence of this part was confirmed by Sanger Sequencing.

Sequence Annotation Location Component / Role(s)
Phytobrick Adapter Site A
ErmE
Phytobrick Adapter Site B
1,4
5,70
71,74
feature/misc sequence_feature
promoter feature/promoter
feature/misc sequence_feature
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1968008/1