Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandra Adriana Schiavon Osorio
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-17 10:55:18
PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1968009_sequence (Version 1) |
Description
The Aspergillus a-glucoamylase (GlaA) enzyme is involved in starch utilization. The glaA promoter is induced by starch, maltose, and low concentrations of glucose but repressed by xylose (Fowler, Berka, & Ward, 1990). Basal transcription of glaA requires only 214 bp upstream of the start codon, while a high-level transcription expression requires the upstream region including -562 and -318 bp in the promoter region. The glaA promoters among different Aspergillus species are conserved.Notes
Phytobrick format compatible with MoClo (Overhangs E and C)Source
This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing| Sequence Annotation | Location | Component / Role(s) |
| Phytobrick Adapter Site E PglaA Phytobrick Adapter Site C | 1,4 5,847 848,851 | sequence_feature feature/misc promoter feature/promoter sequence_feature feature/misc |