Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ricardo Camilo Ch??vez Mart??nez
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 07:41:57
Synechocystis L03 Promoter with RBS* (TetR repressible) Phytobrick
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1968020_sequence (Version 1) |
Description
A Promoter library was created for Synechocystis sp. strain PCC 6803, by Huang et al, using the BBa_R0040 promoter as a template. The L03 promoter comes from this library and was selected because it showed a moderate repression and high induction rates in Synechocystis in the presence of the repressor protein TetR.At the same time, this part includes the Rybosome binding site "RBS*", RBS* which was found to be stronger than BBa_B0034 in Synechocystis (Heidorn et al. 2011)
The fact that TetR does not naturally exist in the cyanobacteria adds second layer of control over the independent behaviour of the promoter. Meaning that TetR will only interact with the operator regions in the L03 promoter(Huang et al. 2013b).
Huang, H.-H. et al., 2013. Wide-dynamic-range promoters engineered for cyanobacteria. Journal of Biological Engineering, 7(1), p.10.
Heidorn T, Camsund D, Huang HH, Lindberg P, Oliveira P, Stensj?? K, Lindblad P, 2011. Synthetic biology in cyanobacteria engineering and analyzing novel functions. Methods Enzymol 2011, 497:539???579.
Notes
This part was designed using the Phytobrick standard.Source
The part is based on a Promoter library by Huang et al. 2013,| Sequence Annotation | Location | Component / Role(s) |
| Phytobrick Adapter Site A L03 Promoter Phytobrick Adapter Site C RBS* | 1,4 5,60 76,79 61,75 | feature/misc sequence_feature feature/promoter promoter feature/misc sequence_feature ribosome_entry_site feature/rbs |