Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ricardo Camilo Ch??vez Mart??nez
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 07:57:42
Synechocystis Sca6-2 Promoter with RBS* (LacI repressible) Phytobrick
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1968021_sequence (Version 1) |
Description
The Sca6-2 promoter, comes from Albers and co-workers, rational design approach towards the existing tac promoter. Several iterations were generated by mutating nucleotides in a step-wise manner within the −10 and −35 cis-acting regions of the promoter. This produced a library of several promoters with a dynamic range of expression strength as well as its ability to be repressed, thanks to the lac operator sequence located after the -10 region of the promoter. These were further characterized in Synechocystis sp. PCC 6803 by Stevan et al. showing that in the presence of the repressor protein LacI, the promoter was able to remain highly repressed. Subsequently, in the presence of the iPTG inducer, the promoter was able to express high concentrations of protein (Stevan C. Albers et al. 2015).Albers, S.C., Gallegos, V.A. & Peebles, C.A.M., 2015. Engineering of genetic control tools in Synechocystis sp. PCC 6803 using rational design techniques. Journal of Biotechnology, 216, pp.36???46.
Notes
The Phytobrick Standard was used for this partSource
Stevan et al. Promoter Library| Sequence Annotation | Location | Component / Role(s) |
| Promoter Sca6-2 RBS* Phytobrick Adapter Site A Phytobrick Adapter Site C | 5,102 103,118 1,4 119,122 | feature/promoter promoter ribosome_entry_site feature/rbs feature/misc sequence_feature sequence_feature feature/misc |