BBa_K1969005

BBa_K1969005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1969005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yijie Lai
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-08 09:05:47

CUP1 promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1969005_sequence (Version 1)

Description

This is the full length of CUP1 promoter in yeast Saccharomyces cerevisiae. As the major copper-activated metallothionine in yeast, Cup1p binds and sequesters cuprous copper(I), Cu+, providing the principal method of removing this metal ion from the cell. CUP1 transcription is specifically induced by the copper-dependent transcription activator Cup2p (more commonly known as Ace1p) in response to high levels of copper ions and by Hsf1p in response to heat shock, glucose starvation and oxidation stress. In the presence of copper, Cup1p is also capable of antioxidant activity and thus contributes a significant, albeit minor, role to oxygen radical detoxification, especially in the absence of Cu,Zn-superoxide dismutase Sod1p.

Notes

As the promoter sequence is rather short, we may use recombinase to inset it upstream different coding sequence to control the expression level.

Source

The genomic sequence from Saccharomyces cerevisiae.

Sequence Annotation Location Component / Role(s)
CUP1 promoter
1,235
feature/promoter promoter
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1969005/1