Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yijie Lai
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-14 10:10:44
S. cerevisiae GPA2 with 5 amino acids from human G(s) alpha-subunit
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1969006_sequence (Version 1) |
Description
This is DNA sequence coding for S. cerevisiae guanine nucleotide-binding protein subunit alpha (GPA2) with 5 amino acids at C terminus replaced by corresponding part of human G(s) alpha-subunit (alpha-S1). The strategy of making yeast???human chimeric G-protein, in which the carboxyl-terminal 5 amino acid residues of yeast G alpha subunit are replaced by the equivalent residues from human Gs proteins, has been shown to improve signal transmission in GPCR engineered yeast according to recent literature.Notes
The endogenous G proteins may not be able to couple with the mammalian GPCRs efficiently, so we do the modifications at C terminal residues to yield better results.Source
It's a combination of yeast Gpa2 coding sequence (from genomic DNA of S. cerevisiae), and human G(s) alpha-subunit (from genomic DNA of human).| Sequence Annotation | Location | Component / Role(s) |
| Gpa2 (C terminus truncated) ATG G(s) alpha (C terminus) TAA | 1,1332 1,3 1333,1347 1348,1350 | feature/protein CDS feature/start start_codon feature/protein CDS feature/stop stop_codon |