Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eivind B??e Drejer
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-21 08:10:29
XOR gate like repressor of CI repressor expression
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1970001_sequence (Version 1) |
Description
This part uses deoxyribozyme ribonucleases to degrade CI repressor mRNA, thereby silencing the gene. The deoxyribozyme ribonucleases are present in two copies, where expression of one is controlled by the lacI promoter and the other by the tetR promoter.The regulatory system works like an XOR gate: Inducing the regulator with one, and only one, inducer results in silencing of CI mRNA expression. Inducing both lacI and tetR promoters results in the deoxyribozyme ribonucleases binding to each other through designed complimentary regions, stopping their mRNA degrading activities. Lack of induction of either promoter should prevent either deoxyribozyme ribonuclease from being synthesized by the cell, in turn preventing their degradation of the CI repressor.
Notes
Designing the deoxyribozyme ribonucleases so that they bound more tightly to each other than to CI repressor mRNA was challenging. Additionally, choosing which promoters to place in front of either gene required some thought on desired expression levels and controlability of the system.Source
De novo DNA.| Sequence Annotation | Location | Component / Role(s) |
| reverse transcriptase lacI promoter DNAzyme msDNA tetR promoter misc RNA msDNA DNAzyme reverse transcriptase misc RNA | 410,1372 1,200 314,358 280,390 1399,1452 209,290 1532,1641 1566,1610 1662,2624 1461,1542 | feature/cds CDS feature/promoter promoter feature/misc sequence_feature feature/misc sequence_feature promoter feature/promoter sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature CDS feature/cds feature/misc sequence_feature |