Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eivind B??e Drejer
Date created: 2016-10-16 11:00:00
Date modified: 2016-10-21 08:05:11
Single deoxynucleotide ribonuclease that binds to and degrades CI repressor mRNA
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1970003_sequence (Version 1) |
Description
This gene, which is placed under the control of lacI, is transcribed into a particular type of single stranded DNA called deoxynucleotide ribonuclease. It targets the mRNA of the CI repressor protein, cleaving it after binding. This results in reduced or completely eliminated expression of the CI repressor gene, depending on the strength of the promoter used to control expression.This gene is designed to be used in conjunction with another ribonuclease which targets and degrades CI repressor mRNA. The two genes target different parts of the mRNA. If expressed together, the ribonucleases bind to each other with higher affinity than to the CI repressor mRNA, in theory stopping the degradation of CI repressor mRNA.
Designed to work in conjunction with BBa_K1970002 for XOR gate like repression of CI repressor mRNA.
Notes
Designing recognition sequences that would bind to CI repressor mRNA with high affinity, but to the gene product of BBa_K1970002 with even greater affinity was very challenging.Source
De novo DNA.| Sequence Annotation | Location | Component / Role(s) |
| tetR promoter msDNA DNAzyme reverse transcriptase misc RNA | 1,54 134,243 168,212 264,1226 63,144 | feature/promoter promoter sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc |