BBa_K197002

BBa_K197002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K197002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Patrick Harrigan

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:17

{OprF AtD}

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K197002_sequence (Version 1)

Description

This is an 8-stranded beta barrel, outermembrane protein. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

Pool O09ig57 through O09ig78, assemble by PCA

PCR O09ig57/O09ig78 on PCA reaction        (710, EcoRI/BamHI

Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-B09ig57   {?OprF AtD?}



O09ig57     PCA assembly of {?OprF AtD?} (B09ig57)

CCATAGAATTCATGAGATCTGACTCTAAAATGACTGGCGCTTACGGTC

O09ig58     PCA assembly of {?OprF AtD?} (B09ig57)

AGAGGTAGAGTCAGAGATACCTTTTTCGATCTGACCGTAAGCGCCAGTCATT

O09ig59     PCA assembly of {?OprF AtD?} (B09ig57)

GTATCTCTGACTCTACCTCTGTTGGTCTGATGCTGGGTGGTAACAACAACAA

O09ig60     PCA assembly of {?OprF AtD?} (B09ig57)

TACCTTTCAGGTCAGAGGTAGCGATGTCAACTTTGTTGTTGTTACCACCCAG

O09ig61     PCA assembly of {?OprF AtD?} (B09ig57)

TACCTCTGACCTGAAAGGTAACTCTGCTTACCTGGGTGGTTACGTTAAACAC

O09ig62     PCA assembly of {?OprF AtD?} (B09ig57)

ATACCAGCGATACCACGGAAAGCACCTTTGTCGTGTTTAACGTAACCACCCA

O09ig63     PCA assembly of {?OprF AtD?} (B09ig57)

TTCCGTGGTATCGCTGGTATCGGTATCCAGTACTCTGACTGGGACTCTCACC

O09ig64     PCA assembly of {?OprF AtD?} (B09ig57)

GTTTTCTTTGTAAGAGTCAGACATGGTGTTACGGTGAGAGTCCCAGTCAGAG

O09ig65     PCA assembly of {?OprF AtD?} (B09ig57)

CTGACTCTTACAAAGAAAACTACTCTGACCGTGGTCTGAACATCTACGCAGA

O09ig66     PCA assembly of {?OprF AtD?} (B09ig57)

GACCTTCGGTCAGTTCTTTGGTGTATTTACCCTCTGCGTAGATGTTCAGACC

O09ig67     PCA assembly of {?OprF AtD?} (B09ig57)

CAAAGAACTGACCGAAGGTCTGTTCCTGGAACCGAAAGTTGGTGTTTCTTAC

O09ig68     PCA assembly of {?OprF AtD?} (B09ig57)

GATTCGTTGATAGATTCCTGTTTGATGTAGTCGTAAGAAACACCAACTTTCG

O09ig69     PCA assembly of {?OprF AtD?} (B09ig57)

CAGGAATCTATCAACGAATCTAAAGACAAAGCTCTGGCTCTGGAAGTTGACG

O09ig70     PCA assembly of {?OprF AtD?} (B09ig57)

AACGTTACCAGACAGGGTGTCGAAGTCTTTACCGTCAACTTCCAGAGCCAGA

O09ig71     PCA assembly of {?OprF AtD?} (B09ig57)

ACACCCTGTCTGGTAACGTTGGTCTGGACCTGCGTAAAGAAATCGTTACCGA

O09ig72     PCA assembly of {?OprF AtD?} (B09ig57)

TAACACCAGCGGTCAGGTTGTGTTTACCTTTTTCGGTAACGATTTCTTTACG

O09ig73     PCA assembly of {?OprF AtD?} (B09ig57)

CAACCTGACCGCTGGTGTTAACTACACCCGTATCCTGTCTGGTGCTGACGAA

O09ig74     PCA assembly of {?OprF AtD?} (B09ig57)

GAGTTACCACCGAAGTTGCCAGTCAGGTTGTCTTCGTCAGCACCAGACAGGA

O09ig75     PCA assembly of {?OprF AtD?} (B09ig57)

GGCAACTTCGGTGGTAACTCTTTCGATATTCTGGTTCCGCAGAAAACCAAAG

O09ig76     PCA assembly of {?OprF AtD?} (B09ig57)

GATGTCGTATTTGATACCAACAGAAACGTTGTCTTTGGTTTTCTGCGGAACC

O09ig77     PCA assembly of {?OprF AtD?} (B09ig57)

TTGGTATCAAATACGACATCGAACTGGAAACTGGCGTTATGCTGGGTGCTAA

O09ig78     PCA assembly of {?OprF AtD?} (B09ig57)

CGTTAGGATCCGTAAGAAACTTTAGCACCCAGCATAACGCC

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K197002/1

Member of these Collections

• iGEM Parts Registry