BBa_K197003

BBa_K197003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K197003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Patrick Harrigan

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:17

{cl02365 AtD}

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K197003_sequence (Version 1)

Description

This is an Autotransporter of the AT-1 family from the organism Neisseria meningitidis. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

Pool O09ig79 through O09ig112, assemble by PCA

PCR O09ig79/O09ig112 on PCA reaction        (854, EcoRI/BamHI

Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-B09ig79   {?cl02365 AtD?}



O09ig79     PCA assembly of {?cl02365 AtD?} (B09ig79)

CCATAGAATTCATGAGATCTGAAAACTCTAACGCTCGTA

O09ig80     PCA assembly of {?cl02365 AtD?} (B09ig79)

CACTGACCAACGCCAGTCATCCACATACGAGCGTTAGAGTTTTCA

O09ig81     PCA assembly of {?cl02365 AtD?} (B09ig79)

ATGACTGGCGTTGGTCAGTGGGGTAACCTGGACTACGGTTACTCT

O09ig82     PCA assembly of {?cl02365 AtD?} (B09ig79)

CAGCGTAGAAGTCAACGTCCATGTTAGAGTAACCGTAGTCCAGGT

O09ig83     PCA assembly of {?cl02365 AtD?} (B09ig79)

GGACGTTGACTTCTACGCTGGTCTGGTTGGTGCTGAAATCGACGT

O09ig84     PCA assembly of {?cl02365 AtD?} (B09ig79)

CAGACCAGCTTTGGTGTTTTCGGTAACGTCGATTTCAGCACCAAC

O09ig85     PCA assembly of {?cl02365 AtD?} (B09ig79)

AAAACACCAAAGCTGGTCTGTTCTTCGGTGCTGGTTCTACCCACT

O09ig86     PCA assembly of {?cl02365 AtD?} (B09ig79)

ATTTTACCGTATTCGTGAGCTTTGTAGTGGGTAGAACCAGCACCG

O09ig87     PCA assembly of {?cl02365 AtD?} (B09ig79)

GCTCACGAATACGGTAAAATCGACTCTAACGACCTCCATGTTGGT

O09ig88     PCA assembly of {?cl02365 AtD?} (B09ig79)

CTTTGATGTTAGAAACACCGTACAGACCAACATGGAGGTCGTTAG

O09ig89     PCA assembly of {?cl02365 AtD?} (B09ig79)

CGGTGTTTCTAACATCAAAGACGTTGCTGCTGTTAACTACGGTAT

O09ig90     PCA assembly of {?cl02365 AtD?} (B09ig79)

GTCACGGTCCTGGTTGGTGTGGGTGATACCGTAGTTAACAGCAGC

O09ig91     PCA assembly of {?cl02365 AtD?} (B09ig79)

ACACCAACCAGGACCGTGACGCTAAACGTACCCTGTGGGTTGGTC

O09ig92     PCA assembly of {?cl02365 AtD?} (B09ig79)

GAGGTAGAGTTGTAACCAGAGGTCTGACCAACCCACAGGGTACGT

O09ig93     PCA assembly of {?cl02365 AtD?} (B09ig79)

TCTGGTTACAACTCTACCTCTTACGACGCTAAAATCACCCAGTTC

O09ig94     PCA assembly of {?cl02365 AtD?} (B09ig79)

GTTGGGTGTAAGCACCTTCCAGGAAGAACTGGGTGATTTTAGCGT

O09ig95     PCA assembly of {?cl02365 AtD?} (B09ig79)

GGAAGGTGCTTACACCCAACTCAACAACGACAAATACTCTATCGA

O09ig96     PCA assembly of {?cl02365 AtD?} (B09ig79)

GAGCCAGTTGAAACCAGCGTACGGTTCGATAGAGTATTTGTCGTT

O09ig97     PCA assembly of {?cl02365 AtD?} (B09ig79)

ACGCTGGTTTCAACTGGCTCCATGTTTCTACCGACTCTATCAACG

O09ig98     PCA assembly of {?cl02365 AtD?} (B09ig79)

GAGAACTGCATGTTACCAACCTGTTCGTTGATAGAGTCGGTAGAA

O09ig99     PCA assembly of {?cl02365 AtD?} (B09ig79)

GTTGGTAACATGCAGTTCTCTACCAAAACCGACAACCAGGACATC

O09ig100     PCA assembly of {?cl02365 AtD?} (B09ig79)

CACGCAGACCGATAGTGCCAACCTGGATGTCCTGGTTGTCGGTTT

O09ig101     PCA assembly of {?cl02365 AtD?} (B09ig79)

TGGCACTATCGGTCTGCGTGGTGGTTACCCGTTCACCGCTGGTTC

O09ig102     PCA assembly of {?cl02365 AtD?} (B09ig79)

GTCACCTTTCAGAGCCATGTTGATAGAACCAGCGGTGAACGGGTA

O09ig103     PCA assembly of {?cl02365 AtD?} (B09ig79)

ACATGGCTCTGAAAGGTGACGTTTCTGCTTCTCACCTGTTCGGTG

O09ig104     PCA assembly of {?cl02365 AtD?} (B09ig79)

TAGAGTTGAGCTTCCGGACGGTTGTCACCGAACAGGTGAGAAGCA

O09ig105     PCA assembly of {?cl02365 AtD?} (B09ig79)

CGTCCGGAAGCTCAACTCTACCTGTCTAACTCTGGTGAAGCTACC

O09ig106     PCA assembly of {?cl02365 AtD?} (B09ig79)

GGTTGTCCAGTTTACCACCACGCAGGGTAGCTTCACCAGAGTTAG

O09ig107     PCA assembly of {?cl02365 AtD?} (B09ig79)

TGGTGGTAAACTGGACAACCTGTTCGGTGTTGGTCTGGGTGTTGA

O09ig108     PCA assembly of {?cl02365 AtD?} (B09ig79)

GGTGGTAGATTTGTTGAGTTGAGCGTCAACACCCAGACCAACACC

O09ig109     PCA assembly of {?cl02365 AtD?} (B09ig79)

AACTCAACAAATCTACCACCTTCGGTGTTTCTTACCAGGGTCAGT

O09ig110     PCA assembly of {?cl02365 AtD?} (B09ig79)

CCAGAAGAAGAAACGTCAGAGTTGTACTGACCCTGGTAAGAAACA

O09ig111     PCA assembly of {?cl02365 AtD?} (B09ig79)

TCTGACGTTTCTTCTTCTGGTATCAACGCTACCCTGAAAGTTAAC

O09ig112     PCA assembly of {?cl02365 AtD?} (B09ig79)

CGTTAGGATCCGAAGTTAACTTTCAGGGTAGCGT

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K197003/1

Member of these Collections

• iGEM Parts Registry