BBa_K197004

BBa_K197004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K197004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Patrick Harrigan

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:18

{VtaA11 AtD}

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K197004_sequence (Version 1)

Description

This is an Autotransporter of the AT-2 family from the organism Haemophilus parasuis. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

Pool O09ig113 through O09ig120, assemble by PCA

PCR O09ig113/O09ig120 on PCA reaction        (269, EcoRI/BamHI

Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-B09ig113   {?VtaA11 AtD?}



O09ig113     PCA assembly of {?VtaA11 AtD?} (B09ig113)

CCATAGAATTCATGAGATCTAACAACAAAGCTCTGCGTGCTGGTATCG

O09ig114     PCA assembly of {?VtaA11 AtD?} (B09ig113)

CCTGCGGCAGACCAGCAGCAGCGTTAGAACCAGCGATACCAGCACGCAGAGCT

O09ig115     PCA assembly of {?VtaA11 AtD?} (B09ig113)

TGCTGCTGGTCTGCCGCAGGTTTATATTCCGGGTAAATCTATGGTTGCTGTTT

O09ig116     PCA assembly of {?VtaA11 AtD?} (B09ig113)

CCAGAGCAGACTGACCTTTGAAAGTGCCAGCAGAAACAGCAACCATAGATTTA

O09ig117     PCA assembly of {?VtaA11 AtD?} (B09ig113)

CAAAGGTCAGTCTGCTCTGGCTGTTGGTTACTCTCGTGCTTCTGACAACGGTA

O09ig118     PCA assembly of {?VtaA11 AtD?} (B09ig113)

TGTTAGCGTTACCTTGGAGTTTCAGGATCAGTTTACCGTTGTCAGAAGCACGA

O09ig119     PCA assembly of {?VtaA11 AtD?} (B09ig113)

ACTCCAAGGTAACGCTAACACCTCTGGTGAAATGGGTGGTTCTGTTGGTGTTG

O09ig120     PCA assembly of {?VtaA11 AtD?} (B09ig113)

CGTTAGGATCCCCACTGGTAACCAACACCAACAGAACCACCC

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K197004/1

Member of these Collections

• iGEM Parts Registry