BBa_K197005

BBa_K197005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K197005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Patrick Harrigan

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:18

{Hag AtD}

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K197005_sequence (Version 1)

Description

This is a 200kDa protein with 10-stranded beta barrel. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

Pool O09ig121 through O09ig128, assemble by PCA

PCR O09ig121/O09ig128 on PCA reaction        (269, EcoRI/BamHI

Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-B09ig121   {?Hag AtD?}



O09ig121     PCA assembly of {?Hag AtD?} (B09ig121)

CCATAGAATTCATGAGATCTAACGAAAACAAAGCTAACGCTGGTATCT

O09ig122     PCA assembly of {?Hag AtD?} (B09ig121)

CCTGCGGCATAGAAGCCATAGCCATAGCAGAAGAGATACCAGCGTTAGCTTTG

O09ig123     PCA assembly of {?Hag AtD?} (B09ig121)

TATGGCTTCTATGCCGCAGGCTTATATTCCGGGTCGTTCTATGGTTACTGGCG

O09ig124     PCA assembly of {?Hag AtD?} (B09ig121)

CAACAGCACCCTGACCGTTGTGGGTAGCGATACCGCCAGTAACCATAGAACGA

O09ig125     PCA assembly of {?Hag AtD?} (B09ig121)

CAACGGTCAGGGTGCTGTTGCTGTTGGTCTGTCTAAACTGTCTGACAACGGTC

O09ig126     PCA assembly of {?Hag AtD?} (B09ig121)

TGTCAGCAGAACCGTTGATTTTGAAAACCCACTGACCGTTGTCAGACAGTTTA

O09ig127     PCA assembly of {?Hag AtD?} (B09ig121)

AATCAACGGTTCTGCTGACACTCAAGGCCATGTAGGTGCTGCTGTTGGTGCTG

O09ig128     PCA assembly of {?Hag AtD?} (B09ig121)

CGTTAGGATCCGAAGTGGAAACCAGCACCAACAGCAGCACCT

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K197005/1

Member of these Collections

• iGEM Parts Registry