BBa_K197007

BBa_K197007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K197007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Patrick Harrigan

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:18

{Hia AtD}

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K197007_sequence (Version 1)

Description

Autotransporter from Haemophilus influenzae. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

Pool O09ig137 through O09ig144, assemble by PCA

PCR O09ig137/O09ig144 on PCA reaction        (269, EcoRI/BamHI

Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-B09ig137   {?Hia AtD?}



O09ig137     PCA assembly of {?Hia AtD?} (B09ig137)

CCATAGAATTCATGAGATCTCTGCGTCGTGAAACCTCTGGTGGTGTTG

O09ig138     PCA assembly of {?Hia AtD?} (B09ig137)

CCTGCGGCAGGTTAGCGGTAGCGATAGCAGCAGCAACACCACCAGAGGTTTCA

O09ig139     PCA assembly of {?Hia AtD?} (B09ig137)

TACCGCTAACCTGCCGCAGGCTTACGTTCAGGGTCGTGGTATGACCTCTGTTG

O09ig140     PCA assembly of {?Hia AtD?} (B09ig137)

CGATAGCAGACTGACCCTGGTAAGAAGAAACACCAACAGAGGTCATACCACGA

O09ig141     PCA assembly of {?Hia AtD?} (B09ig137)

CCAGGGTCAGTCTGCTATCGCTGTTGGTGTTTCTGCTGTTTCTGAATCTGGTC

O09ig142     PCA assembly of {?Hia AtD?} (B09ig137)

TGTTAGCAGAACCAGAGAATTTGAAAACCCAGTGACCAGATTCAGAAACAGCA

O09ig143     PCA assembly of {?Hia AtD?} (B09ig137)

ATTCTCTGGTTCTGCTAACACCCGTTCTCACGTTGGTGTTGGTGCTGGTGTTG

O09ig144     PCA assembly of {?Hia AtD?} (B09ig137)

CGTTAGGATCCCCATTGATAGCCAACACCAGCACCAACACCA

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K197007/1

Member of these Collections

• iGEM Parts Registry