BBa_K197013

BBa_K197013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K197013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Gabriela Guzman Lopez Aguado

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:18

{YuaQ AtD (putative)}

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K197013_sequence (Version 1)

Description

Features: bears sequence similarity to the confirmed autotransporters AIDA and Ag43. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

PCR O09ig306/O09ig305 on MG1655(ask chris about EcoRV before start)  (464bp, gp = frag1)

PCR O09ig304/O09ig307 on MG1655(ask chris about EcoRV before start)  (452bp, gp = frag2)



PCR O09ig306/O09ig307 on mixed frags    (898bp, EcoRI/BamHI)

Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-B09ig306         {?yuaQ AtD?} 



O09ig306    Forward PCR of {?yuaQ AtD?}  (B09ig306)

AAGTGGAATTCATGAGATCTGGAGAACCGAAAGCAACCAG

O09ig305    Reverse internal oligo for {?yuaQ AtD?}  (B09ig306)

GCTGCTGAACTCCCCGATTTTC

O09ig304    Forward internal oligo for {?yuaQ AtD?}  (B09ig306)

GAAAATCGGGGAGTTCAGCAGC

O09ig307    Reverse PCR of {?yuaQ AtD?}  (B09ig306)

GTTAGGGATCCGAATGTATACTTCACACCCAG

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K197013/1

Member of these Collections

• iGEM Parts Registry