BBa_K197015

BBa_K197015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K197015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Gabriela Guzman Lopez Aguado

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:18

{eCPX (circularly permuted OmpX)}

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K197015_sequence (Version 1)

Description

Features: protein is an enhanced CPX variant located in the outermembrane that joins the N- and C-termini of OmpX. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

 

PCR ca998/O09ig282 on M10014 	(477, EcoRI/BamHI)

Sub in pBca9523-Bca1144#5           	(EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-O09ig282   	{}



Ca998  	forward oligo 

gtatcacgaggcagaatttcag

O09ig282 	reverse oligo with stop codons removed for {}

ccggtGGATCCAGAGCTTGCAGTACGGCTTT

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K197015/1

Member of these Collections

• iGEM Parts Registry