BBa_K197020

BBa_K197020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K197020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gabriela Guzman Lopez Aguado
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:18

strep



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K197020_sequence (Version 1)

    Description

    The streptavidin peptide binds to a biotin like protein and this can be used for tagging and purification.

    Notes

    N/A

    Source

    PCR ca998/ca1363R on pBca9145-Bca9494 (1539 bp, EcoRI/BamHI)
    Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
    Product is pBca9495CA-Bca1363 {Pbad.rbs.pp.ST}

    ca998 Forward Sequencing of pSB1A2/pSB1A3
    gtatcacgaggcagaatttcag
    ca1363R Reverse StrepTag
    ccataGGATCCctgcccagactgCCCTCCCTC

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K197020/1
     

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