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BBa_K1972012

BBa_K1972012 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K1972012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shuli Liang

Date created: 2016-09-15 11:00:00

Date modified: 2016-09-16 09:22:38

dszBCAD with T7 promoter

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K1972012_sequence (Version 1)

Description

We constructed dszBCAD genes with T7 promoter and choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.

Notes

No.

Source

4 enzyme genes was systhsized respectively and we construted them to one cassette.

Components

Access	Instance	Definition
public public public public public public public	BBa_I712074 BBa_J61100 BBa_K1972001 BBa_K1972002 BBa_K1972000 BBa_K1972003 BBa_B0012	BBa_I712074 BBa_J61100 BBa_K1972001 BBa_K1972002 BBa_K1972000 BBa_K1972003 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I712074 BBa_J61100 BBa_K1972001 BBa_K1972002 BBa_K1972000 BBa_K1972003 BBa_B0012	1,46 55,66 73,1170 1177,2430 2437,3798 3805,4383 4392,4432	BBa_I712074 BBa_J61100 BBa_K1972001 BBa_K1972002 BBa_K1972000 BBa_K1972003 BBa_B0012