BBa_K1973013

BBa_K1973013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1973013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Claret Fern??ndez
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 11:51:21

Pm (1)



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1973013_sequence (Version 1)

Description

Pm1 BBa_K1973013 Pm1 is one variant of the promoter for the xylS/Pm expression system. It was obtained by site-directed mutagenesis PCR from the Pseudomonas putida plasmid pMPO52 in order to remove illegal restriction sites. The expression of this promoter is activated by XylS or XylS2 in the presence of benzoate and salicylate, respectively.

Notes

It did have to be site-directed mutagenized in position 65 in order to remove a PstI restriction site.

Source

It was obtained by PCR from a plamid that had its sequence.

Sequence Annotation Location Component / Role(s)
Pm (1)
A -> T mutation
1,388
65,65
feature/promoter promoter
sequence_alteration feature/mutation
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1973013/1