BBa_K1973015

BBa_K1973015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1973015
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Claret Fern??ndez
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 11:58:27

Pm (3)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1973015_sequence (Version 1)

Description

Pm3 is one variant of the promoter for the xylS/Pm expression system. It was obtained by site-directed mutagenesis PCR from the Pseudomonas putida plasmid pMPO52 in order to remove illegal restriction sites. The expression of this promoter is activated by XylS or XylS2 in the presence of benzoate and salicylate, respectively.

Notes

It had to be site-directed mutagenized at position 65 in order to remove a PstI restriction site.

Source

It was obtained from a plasmid containing its sequence.

Sequence Annotation Location Component / Role(s)
Pm (3)
A -> C mutation
1,388
65,65
promoter feature/promoter
feature/mutation sequence_alteration
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1973015/1