BBa_K1973028

BBa_K1973028 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1973028
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jos?? Mart??n G??mez
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 03:32:52

miniTn7BBGm-nahR-Psal-nasF-glpF



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1973028_sequence (Version 1)

Description

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. glpF (BBa_K1973026) is expressed from the Psal promoter in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).

Notes

This composite part was created by cloning the parts BBa_K510000, BBa_K1973033, BBa_K1973001 and BBa_K1973026 together.

Source

This composite part was created by cloning the parts BBa_K510000, BBa_K1973033, BBa_K1973001 and BBa_K1973026 together.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1973028/1