Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jos?? Mart??n G??mez
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 03:54:48
miniTn7BBGm-nahR-Psal-nasF-epi
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1973032_sequence (Version 1) |
Description
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. epi (BBa_K1973030) is expressed from the Psal promoter in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).Notes
This composite part was created by cloning the parts BBa_K510000, BBa_K1973033, BBa_K1973001 and BBa_K1973030 together.Source
This composite part was created by cloning the parts BBa_K510000, BBa_K1973033, BBa_K1973001 and BBa_K1973030 together.| Access | Instance | Definition |
| public public public public | BBa_K510000 BBa_K1973033 BBa_K1973001 BBa_K1973030 | BBa_K510000 BBa_K1973033 BBa_K1973001 BBa_K1973030 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510000 BBa_K1973033 BBa_K1973001 BBa_K1973030 | 1,4388 4397,5435 5444,5547 5556,6075 | BBa_K510000 BBa_K1973033 BBa_K1973001 BBa_K1973030 |