BBa_K1974003

BBa_K1974003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1974003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: YU-CHUN WU
Date created: 2016-10-12 11:00:00
Date modified: 2017-03-28 09:53:57

Orally Active Insecticidal Peptide (OAIP)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1974003_sequence (Version 1)

Description

This year we create a revolutionary system that integrates biological pesticides, automatic detector, sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. Orally Active Insecticidal Peptide is coded for the venom of a spider, Selenotypus plumipes. It is under the control of the strong T7 promoter. A 6xHistag is added for further protein purification.

Mechanism of OAIP
Orally Active Insecticidal Peptide has a structure called ICK(inhibitor cysteine knot). This kind of structure contains three disulfide bonds. With this structure OAIP can resist the high temperature, acid base solution and the digest juice of insect gut. OAIP can bind on the voltage-gated sodium channel in the insect???s nervous system, making it paralyze and die eventually.

Notes

It is under the control of the strong T7 promoter. A 6xHistag is added for further protein purification.

Source

Artificial Synthesis

igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1974003/1