BBa_K1975005

BBa_K1975005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1975005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joachim Steen Larsen

Date created: 2016-10-04 11:00:00

Date modified: 2016-10-05 06:40:52

Cyan Fluorescent Protein

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1975005_sequence (Version 1)

Description

This is Cyan Fluorescent Protein that can be used to tag proteins of interest. It is a fluorescent protein that will show a blue color when it is excited at 405nm and it has the highest absorbance at 485nm. This sequence has been used to tag PhaA to visualize the expression of the protein. If the protein was expressed correctly, the cells would show a blue color when excited.
The sequence is optimized for use in Bacillus subtilis.

Notes

To secure the optimal expression we choose to make a codon optimization of the genes. This was done through the Codon Optimization tool at IDT's webpage.

Source

The sequence for CFP genomic DNA from Acropora aculeus. This sequence has also been optimized for expression in B. subtilis.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1975005/1

Member of these Collections

• iGEM Parts Registry