| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1975006_sequence (Version 1)
|
Description
This is Red Fluorescent Protein that can be used to tag proteins of interest. It is a fluorescent protein that will show a red color when it is excited at 558nm and it has the highest emission at 583nm. This sequence has been used to tag PhaB to visualize the expression of the protein. If the protein was expressed correctly, the cells would show a red color when excited. The sequence is optimized for use in Bacillus subtilis.
Notes
To secure the optimal expression we choose to make a codon optimization of the genes. This was done through the Codon Optimization tool at IDT's webpage.
Source
This sequence originally origin from the red sea anemone Discosoma. However, this sequence has been optimized to be used in Bacillus subtilis.
